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Cell Cytometry and Microscopy Unit

Flow cytometry has signified critical advances in multiple areas: clinics. Clinicla research, biomedical research and industry. The possibility of performing parametric analysis on specific celular populations allows the precise and fast identification of such populations. Cell sorting conjugates the power of multiparametric analysis with selection and sorting of a specific cell type to be further used in other applications such as: cell culture, PCR, western blot, kinetic determinations, etc..

Flow cytometers, with or without sorting capabilities, use a laser beam to intercept a single cell generating light dispersión conditined by: cell size, cell granularity and, by using specific antibodies coupled to flourochromes, allow the determination of specfic markers in the cells. Cell sorters utilize this information to sellect cell populations according to the desired criteria and sort them adjusting recovery efficiency and/or specificity. Mordern sorter cytometers allow the separation of isolated single cells unsign edicated clonning modules.

Cell Cytometry and Microscopy Unit is located within the premises of Inbiomed and started operations in the Summer of 2010. This unit allows in house and outside researchers and clinitians multiparametric flow cytometry analysis and cell sorting of selected cell populations based on mollecular phenotype. This unit counts with state of the art cell sorting equipment, magnetic cell separador, 2 flow cytometry analyzers and a confocal microscope.

Technology Support

The Unit offers cell sorting service managed and executed exclusively by the unit trained staff. Analytical flow cytometry and Confocal Microscopy is operated on a user based manner for experienced users.

Training

The Cell Cytometry and Microscopy Unit offers continuos training for interested users in this technology as well as on-demand training, monographic courses and seminars, both for in house researchs and for the public. Detailed schedule of programmed activities will be available shortly.

Researchers interested in direct consulting for flow cytometry and/or cell sorting should contact the Unit Head.

Research

This unit is set as a technology service for research support to Inbiomed research programs and outside parties. As such it is focused on the continious implementation and optimization of new protocols and techniques that become available that may be relevant for stem cell research.

Equipment

• Flow Cytometers:
  • FACScalibur (Becton Dickinson): Argon laser (488 nm) and laser dioide (635 nm) for simultaneous 4-color reading.
  • FACS Canto A (Becton Dickinson): Argon laser (488 nm) and laser dioide (635 nm) for simultaneous 6-color reading.
• Cell Sorters:
  • FACSAria III (Becton Dickinson): Red laser (633 nm) and Blue laser (488 nm) with ACDU (Automatic Cell Deposition Unit) with 4-way simultaneous sorting.
  • AutoMACS (Miltenyi Biotec): magnetic cell separator.
• Confocal Microscopy:
  • Zeiss LSM510 Meta: 3 laser setup (488, 550 and 633) and UV laser. Incubation system for life cell work. Transmited light and CCD camera.

Staff

Unit Head: María Paz López Mato (mplopez@inbiomed.org)
Technical support: Ariane Aduriz Berasategui.